The pI-trap utilizes a series of vessels with a capillary passing through each vessel. Part of the capillary in each vessel is replaced by a semipermeable membrane. The vessels contain buffer solutions with different pH values, so that a pH gradient is effectively established inside the capillary...
The pI-trap utilizes a series of vessels with a capillary passing through each vessel. Part of the capillary in each vessel is replaced by a semipermeable membrane. The vessels contain buffer solutions with different pH values, so that a pH gradient is effectively established inside the capillary. The buffer solution in each vessel has an electrical contact with a voltage divider connected to a high-voltage power supply, which established electrical field along the capillary. When a sample (a mixture of peptides or proteins) is injected in the capillary, the presence of the electrical field and the pH gradient n separates these molecules according to their isoelectric points (pI).
Heart of the device is the pI-Cell, based on proprietary microfluidic technology. Take it as an electrical column, capturing charged molecules according to their isoelectric point.
https://en.wikipedia.org/wiki/Isoelectric_focusing
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